e coli atcc 25922gfptm  (ATCC)

Journal: Antibiotics

Article Title: Using GFP-Tagged Escherichia coli to Investigate the Persistence of Fecal Bacteria in Vegetated Wetlands: An Experimental Approach

doi: 10.3390/antibiotics9060335

Figure Lengend Snippet: Experimental design. Planted and unplanted rhizotrons inoculated with GFP- Escherichia coli in water or sediment.

Article Snippet: We used a fluorescent and nonpathogenic strain E. coli GFP ATCC ® 25922GFPTM (American Type Culture Collection, Manassas, VA, USA).

Techniques: Control

Journal: Antibiotics

Article Title: Using GFP-Tagged Escherichia coli to Investigate the Persistence of Fecal Bacteria in Vegetated Wetlands: An Experimental Approach

doi: 10.3390/antibiotics9060335

Figure Lengend Snippet: Effect of the type of inoculation—in water ( n = 6) or in sediment ( n = 6)—on the fluorescence of GFP- Escherichia coli in the rhizotrons. Boxplots of fluorescences measured on days 1, 2, 3, 6 and 8 (fluorescences > limit od detection (LOD)) in the water of rhizotrons inoculated with GFP- E. coli . Using an ANOVA with a multiple comparisons Tukey’s test on the l-mm, testing the effects of the type of inoculation on the fluorescence (i.e., bacterial concentration) of the water column. a and b are significantly different ( p -value (ANOVA) < 0.01). CTCF: corrected total cell fluorescence.

Article Snippet: We used a fluorescent and nonpathogenic strain E. coli GFP ATCC ® 25922GFPTM (American Type Culture Collection, Manassas, VA, USA).

Techniques: Fluorescence, Concentration Assay

Journal: Antibiotics

Article Title: Using GFP-Tagged Escherichia coli to Investigate the Persistence of Fecal Bacteria in Vegetated Wetlands: An Experimental Approach

doi: 10.3390/antibiotics9060335

Figure Lengend Snippet: Effects of Baldellia ranunculoides ( n = 3), Elodea canadensis ( n = 3), Mentha aquatica ( n = 3), Sparganium emersum ( n = 3), the mixture of these four species together ( n = 3) and the absence of plants ( n = 3) on the fluorescence of GFP- E. coli in the rhizotrons. Boxplots of fluorescences measured on days 1, 2, 3, 6 and 8 (fluorescences > LOD) in the water of rhizotrons inoculated with GFP- E. coli . Using an ANOVA with a multiple comparisons Tukey’s test on the l-mm, testing the effects of plant species on the fluorescence (i.e., bacterial concentration) of the water column. a and b are significantly different ( p -value (ANOVA) < 0.001). The dots are outliers.

Article Snippet: We used a fluorescent and nonpathogenic strain E. coli GFP ATCC ® 25922GFPTM (American Type Culture Collection, Manassas, VA, USA).

Techniques: Fluorescence, Concentration Assay

Journal: Antibiotics

Article Title: Using GFP-Tagged Escherichia coli to Investigate the Persistence of Fecal Bacteria in Vegetated Wetlands: An Experimental Approach

doi: 10.3390/antibiotics9060335

Figure Lengend Snippet: Example of planted rhizotrons after the inoculation of fluorescent E. coli (3rd day after inoculation). ( a ) Rhizotron under natural light. ( b ) Rhizotron under fluorescence conditions (enlightened only with a 480–530-nm light and observed with an amber filter). Photo obtained with an exposition of 13 s f/18 and ISO 800 with a Canon EOS 7D.

Article Snippet: We used a fluorescent and nonpathogenic strain E. coli GFP ATCC ® 25922GFPTM (American Type Culture Collection, Manassas, VA, USA).

Techniques: Fluorescence

Journal: Antibiotics

Article Title: Using GFP-Tagged Escherichia coli to Investigate the Persistence of Fecal Bacteria in Vegetated Wetlands: An Experimental Approach

doi: 10.3390/antibiotics9060335

Figure Lengend Snippet: Fitting curve between the fluorescence measurements (CTCF) and the bacterial concentration (McF). Twelve vials with known concentrations of GFP- E. coli (0.2, 0.4, 0.5, 0.7, 0.8, 0.9, 1, 1.5, 2, 3, 4 and 6 McF) were photographed three times under the same conditions as the rhizotrons. The values presented are the means of these three measurements for each vial.

Article Snippet: We used a fluorescent and nonpathogenic strain E. coli GFP ATCC ® 25922GFPTM (American Type Culture Collection, Manassas, VA, USA).

Techniques: Fluorescence, Concentration Assay